Technique (HRIM-MS)
Hurrah for HRIM
Based on SLIM digital separation technology, high-resolution ion mobility (HRIM) enables best-in class biomolecule separation. HRIM separates and characterizes complex molecules with single-pass resolving power of >250 — providing higher resolution than current ion mobility or liquid chromatography (LC) separation techniques.
Incorporating HRIM into traditional analytical workflows:
- Increases reproducibility based on gas phase separations
- Improves confidence in separation data
- Improves baseline separation of isomeric and isobaric analytes
- Reduces dependence on separation by liquid chromatography
The Challenge
To combat disease, we need to look beyond genomics to the study of proteins, lipids, metabolites and other molecules that have the potential to be more indicative of disease.
In absence of high-resolution and throughput, pharmaceutical and clinical researchers struggle to identify clinically significant molecules when using existing separations technologies. These deficiencies limit the characterization of biologic therapeutics and slow biomarker discovery which are crucial to the development of more effective therapeutics and diagnostic assays.
Achieve Next-Level Separation
Separate in the Gas Phase
HRIM separates ions based on the difference in collision cross section (CCS), which incorporates size, charge density, and overall shape. By doing this in the gas phase, results are fast and highly reproducible.
How does it work? Conventional electrospray or other ionization methods disperse the sample. As the ions enter the separation chamber, voltages applied to electrodes on printed circuit boards (PCBs) provide the lossless separation conduit through which the ions travel the separation path length. Smaller molecules undergo fewer collisions with the buffer gas than larger molecules, so they travel faster — arriving at the detector first, achieving separation.
Separate with Confidence
HRIM allows more predictable and reproducible results by separating based on physical parameters of the analytes themselves. HRIM does not make use of specific columns or solvent systems, and the process for running HRIM requires much less user input, so there are fewer variables to control. This makes molecule separations compound class agnostic.
Separate from the Noise
HRIM filtering capabilities improve signal-to-noise with easy separation of analytes and matrix interferences over a broad mass range.
Separate with Unprecedented Resolution
In situations where multiple molecules have similar elution profiles, LC separations alone can take more than an hour to resolve them effectively, if at all. Add HRIM to a workflow, and the additional 13 meters of separation path provide resolving powers of >250 across the entire mass range without the need to zoom in on areas of interest — enabling separation of even the most challenging molecules in a few minutes or less.
Separate Yourself from the Bench
Running samples of varying analytes back-to-back is practical and simple, significantly increasing lab productivity. HRIM allows for the separation of multiple classes of analytes without component changes. With no hardware, column, or mobile phase changes to make, the same instrument can be used across the board.
Separate Without Compromise
HRIM eliminates the common trade-offs between speed, resolution, and reproducibility. Digitized molecule separations in the gas phase do not suffer the inherent drawbacks of traditional mass spectrometry methods. HRIM empowers fast, accurate, and consistent characterization of multiple analytes.
Separate in the Gas Phase
HRIM separates ions based on the difference in collision cross section (CCS), which incorporates size, charge density, and overall shape. By doing this in the gas phase, results are fast and highly reproducible.
How does it work? Conventional electrospray or other ionization methods disperse the sample. As the ions enter the separation chamber, voltages applied to electrodes on printed circuit boards (PCBs) provide the lossless separation conduit through which the ions travel the separation path length. Smaller molecules undergo fewer collisions with the buffer gas than larger molecules, so they travel faster — arriving at the detector first, achieving separation.
Separate with Confidence
HRIM allows more predictable and reproducible results by separating based on physical parameters of the analytes themselves. HRIM does not make use of specific columns or solvent systems, and the process for running HRIM requires much less user input, so there are fewer variables to control. This makes molecule separations compound class agnostic.
Separate from the Noise
HRIM filtering capabilities improve signal-to-noise with easy separation of analytes and matrix interferences over a broad mass range.
Separate with Unprecedented Resolution
In situations where multiple molecules have similar elution profiles, LC separations alone can take more than an hour to resolve them effectively, if at all. Add HRIM to a workflow, and the additional 13 meters of separation path provide resolving powers of >250 across the entire mass range without the need to zoom in on areas of interest — enabling separation of even the most challenging molecules in a few minutes or less.
Separate Yourself from the Bench
Running samples of varying analytes back-to-back is practical and simple, significantly increasing lab productivity. HRIM allows for the separation of multiple classes of analytes without component changes. With no hardware, column, or mobile phase changes to make, the same instrument can be used across the board.
Separate Without Compromise
HRIM eliminates the common trade-offs between speed, resolution, and reproducibility. Digitized molecule separations in the gas phase do not suffer the inherent drawbacks of traditional mass spectrometry methods. HRIM empowers fast, accurate, and consistent characterization of multiple analytes.
Featured Resources
Schedule a Chat Today!
