Peptide Characterization
Unravel the Complexity of Peptide Analysis
Changes in peptides can effect the safety and performance of therapeutics, making structural analysis a critical step in drug development.
The Problem
PTM and Impurity detection with MOBIE
Scientists need the ability to identify and measure peptide modifications, structure, and impurities more definitively.
With liquid chromatography-mass spectrometry (LC-MS), lack of resolution lets some deamidation and isomerization events go undetected. Where the resolution is high enough, quantitative analysis can take over an hour.
Our Solution
Current analytical strategies for Peptide Therapeutics fail to adequately identify insertions, deletions and substitutions that have small or no mass changes.
Combining the MOBIE® platform’s high-resolution ion mobility mass spectrometry (HRIM-MS) with liquid chromatography more accurately separates and identifies impurities and post-translational modifications (PTMs) and reduces run times and costs.
Benefits of HRIM
MOBIE provides improved confidence in identification of difficult to measure PTMs and impurities.
- Identification of mass-neutral PTMs difficult to monitor by LC-MS alone
- Increased throughput for separation, detection, and quantitation of key analytes
- Detection of degradation products during manufacturing
- Reduced dependency on liquid chromatography
Reduce LC run time (left) while maintaining high-resolution separation in the ion mobility dimension (right). Here, the ion mobility data is identical, independent of the LC gradient used.
Customer Success Story
Resolving Complex Lipid Structures
Kim Ekroos PhD - Lipidomics Consulting Inc.
MOBIE achieves separation of molecules with close structural heterogeneity without LC
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