Masked Multiplexed Separations to Enhance Duty Cycle for Structures for Lossless Ion Manipulations
Brian H. Clowers, Elvin Cabrera, Gordon Anderson, Liulin Deng, Kelly Moser, Gregory Van Aken, and John Daniel DeBord
The experimental paradigm of one ion packet release per spectrum severely hinders throughput in broadband ion mobility spectrometry (IMS) systems (e.g., drift tube and traveling wave systems). Ion trapping marginally mitigates this problem, but the duty cycle deficit is amplified when moving to high resolution, long pathlength systems. As a consequence, new multiplexing strategies that maximize throughput while preserving peak fidelity are essential for high-resolution IMS separations [e.g., structures for lossless ion manipulations (SLIMs) and multi-pass technologies]. This paper explores how MOBILion's technology helped obtain a 100-fold increase in ion utilization with HRIM-MS.
The paper, published in Analytical Chemistry presents:
- A simplified approach, using MOBILion's SLIM technology, to develop the masked transform demonstrating clear benefits compared to the traditional transform.
- The opportunity exists to develop customized deconvolution masks tailored to a specific instrumental configuration.
- The study was conducted using a serpentine path [HRIM-MS] SLIM ion mobility spectrometer (MOBILion Systems) integrated with a commercial quadrupole time-of-flight (QTOF) mass spectrometer.
- By enabling the use of higher bit order sequences (e.g., 6, 7, and 8-bit), the duty cycle and ion utilization efficiency are maximized for SLIM with ion enhancement factors approaching 100 compared to the traditional single-release paradigm for data acquisition. The peak widths and the fidelity of arrival time distributions (ATDs) were maintained.
- MOBILlion System helped in this study to leverage a new class of deconvolution strategies that provide access to vastly improved experimental duty cycles while minimizing spectral artifacts that emerge from traditional deconvolution approaches. These developments promise to enhance the utility of SLIM across domains by maximizing ion throughput and sensitivity.
Analytical Chemistry 2021 93 (14), 5727-5734 DOI:10.1021/acs.analchem.0c04799