Application Notes

Comprehensive PFAS Profiling in AFFF-Contaminated Environmental Samples

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Comprehensive PFAS Profiling in AFFF-Contaminated Environmental Samples


Thomas Lubinsky, Rachel Harris, Cassie Eisele, Michelle English, Jeffrey Webber, Tarun Anumol, Frederick G. Strathmann


This application note presents a detailed study on the advanced analysis of Per- and Polyfluoroalkyl Substances (PFAS) in environmental samples. Utilizing High-Resolution Ion Mobility (HRIM) in conjunction with Liquid Chromatography-Mass Spectrometry (LC-MS), it demonstrates enhanced accuracy and separation of PFAS compounds, particularly in distinguishing branched isomers. The integration of HRIM with LC-MS offers a significant improvement in sample characterization, facilitating comprehensive profiling of diverse PFAS compounds and contributing valuable insights into their environmental impact and behavior.

Customer Intro
  • HRIM provides separation of linear sulfonates from their branched forms, reducing error in quantitation caused by coeluting branched forms.
  • HRIM data is robust, providing highly repeatable measurements.
  • Integrating HRIM in LC-MS workflows allows for a greater characterization of branched species LC alone is unable to achieve.
  • PFAS subclasses display characteristic relationships between the increase in the length of the carbon chain and corresponding arrival time, providing additional confidence in prioritization, classification, and annotation of untargeted samples even when standards are lacking.

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