Application Notes

High-Throughput Oligonucleotide Mapping Workflow Enabled by High-Resolution Ion Mobility

Author(s)

Kelly L. Moser, Frederick G. Strathmann

Abstract

Demonstrate the ability of high-resolution ion mobility (HRIM) to enhance oligonucleotide mapping through high-throughput full scan separations and superior isomer resolution.

Customer Intro

Problem

Solution

Summary

Abstract

Monitoring oligonucleotide sequence coverage and modification presence (including isomeric forms) is critical to ensure safety, efficacy, and quality of synthetic RNA therapeutics. A simple 1-minute FIA-HRIM-MS MAF workflow using automated software for data analysis was demonstrated for a modified sgRNA molecule where 98% sequence coverage was achieved using two enzymes for digestion, in addition to the ability to monitor isomeric PS modifications.

Results